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Bio-Expert (Industry Oriented)Computational & Structural Biology (Module 1)
Conceptual understanding of molecular biology and Structural Biology, Sources of biological data: major databases in bioinformatics, data files and analysis. Sequence data, searching and alignment, genome sequencing, genome analysis, genetic variation, gene (Genebank, EMBL, Unigene, Ensemble etc). Computational methods for pathways and system biology (KEGG, BRITE, Genepath and GeNET), Structural Studies (DNA, RNA and Protein): MFold, RNAfold, IFold, PDB. Concept of homology modelling and molecular docking (Patchdoc, Cluspro).
Proteomics (Module 2)
What is proteomics, Application of proteomics, Good laboratory practices (GLP), Basic instrument handling, Concept of basic calculation and reagent preparation, Screening of protein rich sources for protein estimation, Estimation of protein through UV-Visible spectroscopy, standard Curve preparation and Data Interpretation, Extraction of protein from different sources, Different methods of protein precipitation, Reagents preparation for SDS-PAGE- Stacking gel, running gel, sample loading dye, tank buffer, Staining solution, destaining solution, Protein separation through Vertical gel electrophoresis (SDS-PAGE) technique, Staining and destaining of SDS-PAGE gel and observation.
Genomics and Recombinant DNA Technology (Module 3)
Introduction of RDT, Application of RDT, Recent trends and future perspective in RDT, Good laboratory practices (GLP), basic instrument handling, concept of basic calculation, Learn the direct application of PCR for detection of specific pathogen- HPV16,HPV18, MTB, NTM, HBV. perform Agarose gel electrophoresis of the amplified product, Learn the extraction of DNA from Agarose Gel, Thermocycler, conventional PCR, rt-PCR.
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