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Bioinformatics (Module 1)
Introduction to the field of bioinformatics, historical overview and conceptual understanding about molecular biology, Information Search and Data retrieval: electronic libraries, tools for web search and data mining, Bioinformatics applications, bioinformatics tools, server, pipelines and resources, Introduction of databases: major databases in bioinformatics, data management and analysis, Tools for similarity search and alignments: FASTA, BLAST and other program, DNA/RNA/Protein Sequences: alignments and analysis
Molecular Techniques (Module 2)
Basic of Molecular Biology, Good laboratory practices (GLP), Basic instrument handling, Basic concept of Percentage, Basic concept of Molarity and Normality, Micropipette handling, Extraction of genomic DNA from human sample, Extraction of genomic DNA from Virus, Qualitative Analysis of DNA-Agarose Gel Electrophoresis, Quantitative Estimation of the DNA, Learn the direct application of PCR for detection of specific pathogen- HPV16, HPV18, MTB, NTM, HBV. perform Agarose gel electrophoresis of the amplified product, Learn the extraction of DNA from Agarose Gel, Thermocycler, conventional PCR, rt-PCR.
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